Journal: CytoJournal

Article Title: Fibrinogen-like protein 1 induces the formation of an immunosuppressive microenvironment by upregulating fibronectin 1 to promote immune evasion in bladder cancer

doi: 10.25259/Cytojournal_80_2025

Figure Lengend Snippet: Fibrinogen-like protein 1 ( FGL1) expression in bladder cancer tissues and cell lines is upregulated. (a) The messenger RNA expression of FGL1 in adjacent and tumor tissues from bladder cancer patients was detected by reverse transcription quantitative polymerase chain reaction paired sample t -test. n = 30. (b and c) Western blotting was used to detect the protein expression of FGL1 in bladder cancer cell lines including 5637, RT4, T24, and UMUC3, and human ureteral epithelial immortalized cell line SV-HUC-1 was used as a control. n = 3. The difference between groups was analyzed by one-way analysis of variance. (d and e) Immunohistochemical images of FGL1 from adjacent and tumor tissues of three bladder cancer patients. The difference between samples was analyzed by paired sample t-test. ✶ ✶ P < 0.01, ✶ ✶ ✶ P < 0.001. Scale bar = 50 µm.

Article Snippet: Bladder cancer cell lines 5637 (HTB-4), RT4 (HTB-2), T24 (HTB-9), and UMUC3 (CRL-1749) and immortalized human ureteral epithelial cell line SV-HUC-1 (CRL-9621) were acquired from the American Type Culture Collection (Manassas, VA).

Techniques: Expressing, RNA Expression, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot, Control, Immunohistochemical staining

Journal: Open Medicine

Article Title: ARAF regulates malignant progression of bladder cancer through the p38MAPK pathway

doi: 10.1515/med-2026-1422

Figure Lengend Snippet: Expression levels of ARAF in bladder cancer cells. (A) mRNA expression of ARAF in different cell lines. (B and C) Protein expression of ARAF in different cell lines. Data are presented as mean ± SD from three independent biological replicates. Statistical analysis was performed using one-way ANOVA. ***p<0.001, ****p<0.0001 vs. SV-HUC-1.

Article Snippet: The human ureter epithelial immortalized cell line SV-HUC-1 (CL-0222) and human bladder cancer cell lines T24 (CL-0227) and J82 (CL-0125) were purchased from Wuhan Procell Biological Company.

Techniques: Expressing

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: RBM15 drives bladder cancer progression through YTHDF2-dependent m6A-mediated regulation of ZO2

doi: 10.1186/s13046-026-03684-9

Figure Lengend Snippet: RBM15 drives m6A hypermethylation and the malignant progression of BC in vitro. A , B qPCR ( A ) and WB ( B ) analyses showing upregulated RBM15 expression in a panel of BC cell lines compared with the immortalized urothelial cell line SV-HUC-1. C , D Efficient knockdown of RBM15 in T24 and 5637 cells using two independent shRNAs (shRBM15-1 and shRBM15-2), as confirmed by WB ( C ) and qPCR ( D ). E RNA dot blot analysis showing a reduction in global m6A methylation levels upon RBM15 knockdown. Methylene blue (MB) staining served as a loading control. F , G CCK-8 assays showing that RBM15 knockdown significantly inhibited the proliferation of T24 ( F ) and 5637 ( G ) cells. H , I Transwell assays demonstrating that RBM15 knockdown suppressed the migration and invasion of T24 ( H ) and 5637 ( I ) cells. Representative images and the results of the quantitative analysis are shown. Scale bar, 100 × (10× objective × 10× ocular). Three randomly selected fields per sample (100× magnification) were quantified. J , K WB ( J ) and qPCR ( K ) confirmation of successful RBM15 re-expression (RBM15res) in RBM15-knockdown cells. L RNA dot blot analysis showing that the re-expression of RBM15 rescued global m6A methylation levels. M , N CCK-8 assays showing that RBM15 re-expression reversed the inhibition of the proliferation of T24 ( M ) and 5637 ( N ) cells. O , P Transwell assays showing that RBM15 re-expression restored the migratory and invasive capacities of T24 ( O ) and 5637 ( P ) cells. Scale bars are the same as in H–I. ** p < 0.01 and *** p < 0.001

Article Snippet: The immortalized human urothelial cell line SV-HUC-1 was obtained from Procell Life Science (Wuhan, China), and the murine BC cell line MB49 was obtained from Patone Biotech (Shanghai, China).

Techniques: In Vitro, Expressing, Knockdown, Dot Blot, Methylation, Staining, Control, CCK-8 Assay, Migration, Inhibition

Journal: BMC Pediatrics

Article Title: Hepatocyte growth factor blocks epithelial-mesenchymal transition and fibrosis of ureteral epithelial cells in UPJO

doi: 10.1186/s12887-026-06795-6

Figure Lengend Snippet: HGF Inhibits TGFβ-Induced Fibrosis and EMT in SV-HUC-1 Cells. A - B HGF suppressed TGFβ-induced mRNA expression of fibrotic markers (α-SMA, FN1, Col1a1) and reversed EMT marker expression (E-cadherin downregulated; N-cadherin, β-catenin, Vimentin upregulated) in SV-HUC-1 cells; C HGF suppressed TGFβ-induced protein expression of fibrotic markers (α-SMA, FN1, Col1a1, p-SMAD2/3, p-p65) and reversed EMT marker expression (E-cadherin downregulated; N-cadherin, β-catenin, Vimentin upregulated) in SV-HUC-1 cells. Data are presented as the mean ± SEM of three experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, *** p < 0.001, **** p < 0.0001

Article Snippet: Human ureteral epithelial immortalized cell line SV-HUC-1 and primary human ureteral epithelial cells were purchased from Procell (China, Wuhan).

Techniques: Expressing, Marker

Journal: BMC Pediatrics

Article Title: Hepatocyte growth factor blocks epithelial-mesenchymal transition and fibrosis of ureteral epithelial cells in UPJO

doi: 10.1186/s12887-026-06795-6

Figure Lengend Snippet: HGF Attenuates TGFβ-Induced Migration in SV-HUC-1 Cells

Article Snippet: Human ureteral epithelial immortalized cell line SV-HUC-1 and primary human ureteral epithelial cells were purchased from Procell (China, Wuhan).

Techniques: Migration